PLASMID PROFILE OF ANTIBIOTIC RESISTANT SALMONELLA SPECIES ISOLATED FROM POULTRY PRODUCTS

ABSTRACT

The increased prevalence of Salmonella contamination in poultry has gained considerable scientific attention during the last few decades. The research work was done to determine the plasmid profile of antibiotic resistant Species isolated from poultry products. In the reaserch, 24 strains of Salmonella isolated where tested to ten antibiotics using the disc diffusion method, to determine their susceptibility. The zone of inhibition was observed and recorded in percentage. 24 strains showed 100% resistant to septrin, chloramphenicol, streptomycin, tarvid, agumentin, higher resistant was observed against amoxacilin (19%), Gentamicin (91%) and pefloxacin(91%). The isolates showed lower resistant of 66% against sparfloxacin and ciprofloxacin. While 6 isolates was sensitivity against gentamicin, amoxacilin, sparfloxacin, and ciprofloxacin. Six resistant strain of Salmonella isolates was selected at random to determine the plasmid profile. The result obtained showed that 1, 2, 6 in the DNA ladder are positive to plasmid with the molecular weight of 0.5kb – 48.5kb, while 3, 4, 5, are negative to plasmid.

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